γPNA technology is a synthetic, nonionic nucleic acid analogue that adopts a helically pre-organized structure, leading to exceptionally high affinity for complementary DNA and RNA targets. The benefit of γPNA technology is that it can hybridize to nucleic acid targets even in the context of competing secondary structure, including double-stranded DNA. Moreover, our proprietary structure promotes high water solubility and low aggregation and surface adsorption.
Custom Synthesis. We provide custom synthesized γPNA oligomers for a diverse range of applications in biological and biomedical research. We assist our customers in target sequence selection and oligomer design. Available modifications include affinity tags such as biotin, fluorophores such as cyanine dyes and fluorescein, and amino acids. Oligomers are purified by HPLC and characterized by mass spectrometry before shipping to customers as lyophilized powders.
Fluorescent Telo Miniprobes®. The high affinity of γPNA offers the opportunity to use short miniprobes for fluorescence in situ hybridization (FISH) analysis. This is particularly useful for staining of repetitive sequence targets such as telomeric DNA, where a shorter 12mer γPNA can deliver more probes and, therefore, fluorescent dyes, to a telomere of a given length, than a conventional 18mer or 24mer PNA probe. This results in increased telomere brightness and improved detection of critically short telomeres.