Applications

Affinity Capture and Immobilization Reagents

The high affinity of γPNA allows capture, isolation and immobilization of specific DNA and RNA targets as well as their complexes with proteins. The unprecedented ability of γPNA to invade double-stranded DNA without sequence restrictions means that γPNA can be used to capture even the most elusive targets. We offer γPNA affinity reagents labeled with terminal mono- or bis-biotin groups for capture on streptavidin columns or beads.

A challenge of using a high-affinity reagent such as γPNA is to release the captured target after nonspecific components of a complex mixture have been washed away. PNA Innovations’ γPNA capture reagents are designed to allow release under mild conditions simply by adding a competitor strand that displaces the captured target from the original γPNA probe.

γPNAs can also be synthesized with terminal amine or thiol groups to allow immobilization on a variety of surfaces for a diverse range of applications, including microarray analysis and ELISA tests.

Fluorescent in situ hybridization (FISH) 

FISH – Fluorescent in situ hybridization (FISH) probes made of γPNA provide you with access to the most demanding targets such as duplex DNA, rRNA, and short targets such as miRNA and other non-coding RNA.  For targeting duplex DNA and folded RNA no other probe type possesses the universal strand invasion capability of γPNA, or high enough affinity to effectively bind short RNA targets such as miRNA and pre-miRNA.  Studies have shown that γPNA probes as short as 12-mers can be used to quantitatively measure telomeric DNA sequences with greater sensitivity and brightness than traditional PNA or other probes.  For microbiological analysis, rRNA staining of intact bacterial and fungal cells can be performed to determine the presence, identity, and quantity of a particular microorganism or pathogen, directly within complex sample matrices.  If you application involves mRNA or miRNA detection within tissues or cells then you will find γPNA is a superior alternative to LNA or standard DNA probes.  PNA innovations can provide you with γPNA probes labeled with fluorophores of your choice for your single color or multicolor FISH experiments.

Knockdown

Knockdown – The high affinity, specificity, and nuclease stability of γPNA makes it ideal for use as a gene knockdown and modulation reagents. Whether you wish to block mRNA translation, suppress a miRNA, or correct/knockout a splicing event, you should consider exploring γPNA.  Modifications to the γPNA backbone can be made to make them inherently cell-permeable or you can use direct injection or formulate them with standard carriers for efficient cellular uptake.

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